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RESEARCH / LITERATURE

Research Literature

Find research, methods, and reviews across our scientific fields.

One collection · Original publication dates · Authors, journals & DOIs

All publications 147

Duplicate DOIs are merged. This is a selected collection, not an exhaustive bibliography.

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Methods
AAPS Open

Compaction density reveals reversible urea polymorph changes under pressure

Gerrit Vreeman; Changquan Calvin Sun

An AAPS Open study used a compaction simulator to track reversible density changes in urea between 600 and 900 MPa, consistent with its known form I-to-III transformation. The original abstract proposes in-die density monitoring as a material-sparing screen for pressure-induced phase changes. Applicability depends on a sufficiently large density difference; urea is the demonstrated model compound.

Pharmaceutical solid stateFormulation Development & Manufacturing
Read guide →Original paper ↗

DOI: 10.1186/s41120-026-00206-x

Reviews & perspectives
AAPS Open

Perspective maps oligonucleotide conjugation strategies beyond liver targeting

Ya Ying Zheng; Yonger Xue; Xucheng Hou; Yizhou Dong

An AAPS Open perspective surveys conjugation strategies for antisense oligonucleotides and siRNAs, including small-molecule ligands, cell-penetrating peptides, antibodies, and aptamers. The abstract contrasts clinically established GalNAc delivery with emerging approaches to extrahepatic tissues and discusses translation challenges. This is a synthesis of delivery platforms and clinical progress, rather than a new experimental or clinical efficacy study.

Oligonucleotide Therapeutics & Delivery
Read guide →Original paper ↗

DOI: 10.1186/s41120-026-00209-8

Reviews & perspectives
Bioanalysis

Review examines greener solvent systems for bioanalytical microextraction

Styliani Nisyriou; Marianna Ntorkou; Christina Patakidou; Constantinos K Zacharis

Based on the PubMed abstract, a Bioanalysis review examines switchable-hydrophilicity solvents and bio-derived deep eutectic solvents for sample preparation. It discusses extraction performance, matrix effects, reproducibility, and environmental trade-offs, with future emphasis on miniaturization and automation. The review evaluates methodological opportunities rather than validating a specific assay for regulated clinical sample analysis.

Bioanalysis & Biomarkers
Read guide →Original paper ↗

DOI: 10.1080/17576180.2026.2758030

Original research
Nature Communications

AI-driven discovery and engineering of human-derived protein scaffolds for mRNA delivery

Dongmin Xun; Lanqing Li; Jie Zou; Yi Liu; Ziyue Yang; Qianyi Liang; Ke Li; Zhu Zhu; Jicong Zhang; Lijun Lang; Kun Liu; Xinyu Wang; Meilin Tian; Guangyong Chen; Jun Yang; Bolin An; Pheng Ann Heng; Chao Zhong

The DeepDelivery platform screened the human proteome for proteins that could serve as mRNA delivery scaffolds. Twelve of 15 experimentally tested candidates supported particle formation and delivery within a modular system. Engineering TRIM25 domains improved performance. The work establishes a discovery framework in cultured cells, rather than clinical evidence of reduced immunogenicity.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41467-026-79039-x

Methods
International Journal of Pharmaceutics

Simple heat-assisted method for mRNA loading into empty lipid nanoparticles enables robust in vitro and in vivo delivery

Takayuki Miyazaki; Kenji Kubara; Keita Kondo; Hiroshi Kamiyama; Shoki Hara; Jun Moriya; Hiroshi Ishihara; Yuta Suzuki

A post-loading method combined mRNA with preformed empty lipid nanoparticles using heat and acidic conditions, achieving roughly 90% encapsulation. The particles produced protein expression in mouse liver and spleen, although activity was slightly below conventional microfluidic formulations. Greater payload heterogeneity emerged as a potential limitation; the method supports 96-well preparation.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.ijpharm.2026.127599

Original research
Journal of controlled release : official journal of the Controlled Release Society

Overcoming technological and biological barriers to pulmonary mRNA delivery with lipid-polymer hybrid nanoparticles

Biesel A; Shandheep A; Pavlou A; Busker LM; Koch M; Kopliku V; Maisel K; Boese A; Skerra J; Schröder LJ; Neubert L; Schneider-Daum N; Loretz B; Kalinke U; Lehr CM

Lipid-polymer hybrid particles combined polymer stability with lipid-mediated transfection. Hyaluronic acid and polyvinyl alcohol coatings helped preserve particle properties after nebulization, while mucus-covered airway cultures retained measurable transfection. Primary human lung fibroblasts and intratracheally dosed mice provided additional delivery evidence. These results support aerosol formulation development, although intratracheal dosing does not establish the performance of inhaled aerosols in patients.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.jconrel.2026.115509

Reviews & perspectives
Clinical Pharmacology & Therapeutics

Review identifies higher patient exposure for some drugs in chronic inflammation

Johannes Tillil; Sathej Gopalakrishnan; Jennifer Dong; Karthik Venkatakrishnan; Charlotte Kloft; Wilhelm Huisinga; Lena Klopp-Schulze

Based on the article abstract, a review of 20 approved small-molecule medicines for selected chronic inflammatory diseases found more than 25% higher exposure in patients for five medicines, using reported population pharmacokinetic analyses. The authors highlight CYP3A4 and CYP2C19 metabolism. This cross-drug review supports risk assessment, not automatic dose changes for individual patients.

Clinical Pharmacology & Model-Informed Development
Read guide →Original paper ↗

DOI: 10.1002/cpt.70535

Original research
Nucleic Acid Therapeutics

GuNA-modified antisense gapmers show prolonged activity in mouse brain

Takaaki Kawanobe; Ajaya Ram Shrestha; Hideaki Tomita

Based on the PubMed abstract, researchers combined guanidine-bridged nucleic acid and MOE modifications in antisense gapmers, reporting improved knockdown in laboratory and animal experiments. Activity persisted beyond 24 weeks in mouse brain after intracerebroventricular administration. The study also examined immune responses and toxicity; these preclinical results do not establish human safety or efficacy.

Oligonucleotide Therapeutics & Delivery
Read guide →Original paper ↗

DOI: 10.1177/21593337261502560

Original research
Nature Biotechnology

An mRNA–lipid nanoparticle vaccine against H5N1 influenza virus protects lactating dairy cows from infection

Jefferson J. S. Santos; Carine K. Souza; Giovana C. Zanella; Débora B. Goulart; Bailey Arruda; Paola Boggiatto; Mitchell V. Palmer; Celeste A. Snyder; Michaela A. Kristula; Charlene Dickens; Terry L. Webb; Reilly K. Atkinson; Bernadeta Dadonaite; Garima Dwivedi; Mohamad-Gabriel Alameh; Christopher A. Hunter; Lisa A. Murphy; Jesse D. Bloom; Drew Weissman; Gary C. Althouse; Amy L. Baker; Scott E. Hensley

An experimental H5N1 mRNA-LNP vaccine was tested in eight lactating dairy cows split between vaccinated and control groups. After two doses and mammary challenge, vaccination reduced peak viral RNA in milk by about 1,000-fold and lessened mastitis and fibrosis. This small animal study does not establish field effectiveness or human protection.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41587-026-03343-6

Original research
Journal of controlled release : official journal of the Controlled Release Society

Uncovering the lymphatic uptake, pharmacokinetics, and biodistribution of mRNA-lipid nanoparticles after intravenous and subcutaneous injection

Peng Li; Mohammad Abdallah; Yuxin Yao; Rekha Shandre Mugan; Thomas J. Payne; Stewart A. Fabb; Colin W. Pouton; Natalie L. Trevaskis

Using three established ionizable lipids, researchers measured RNA, lipid components, lymphatic transport, plasma exposure, and tissue reporter expression in rats. After subcutaneous injection, lymph concentrations exceeded plasma concentrations, but lipid and RNA trafficking did not fully match. The study demonstrates why tracking a lipid label alone may not accurately represent intact RNA delivery or productive expression in tissues.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.jconrel.2026.115491

Reviews & perspectives
Nature Reviews Materials

Programmable mRNA vaccine materials for temporal control of antitumour immunity

Seong Dong Jeong; Chaoyang Tang; Xiaotian Wang; Antoni J. Szeglowski; Yujin Kim; B. J. Kim; Padmanee Sharma; James J. Moon; Wen Jiang; Betty Y. S. Kim

A Perspective proposes designing mRNA vaccine materials around changing immune conditions before and after cancer surgery. The framework considers a broadly applicable preoperative prime followed by a personalized postoperative boost, with carriers controlling expression and immune activation. It is a conceptual design roadmap, not evidence from a new clinical trial.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41578-026-00974-8

Original research
Molecular therapy : the journal of the American Society of Gene Therapy

A Scalable, Antibody-Free Lipid Nanoparticle Achieves Preferential mRNA Delivery to Human HSPCs In Vivo Without Conditioning

Kim E; Zenhausern R; Kapadia S; Johnson S; Cho S; Choi J; Park K; Diaz E; Podilapu AR; Kuppan R; Dahlman JE; Kim H; Woo J

A ligand-free nanoparticle formulation preferentially delivered mRNA to human hematopoietic stem and progenitor cells in humanized mice without conditioning. It reduced hepatic delivery relative to an SM-102 comparator and enabled functional Cas9-mediated editing of human CD34-positive cells. Single-cell analyses supported tolerability under the conditions examined. Durable stem-cell function and clinical delivery still require further investigation.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.ymthe.2026.10.024

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