Efficient production of a self-amplifying RNA vaccine sequence with the single subunit RNA polymerase from Klebsiella phage 32 utilizing the native AU initiating dinucleotide
Academic source · RNA
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Site published: 2026-11-03 · Updated: 2026-11-03
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What the paper reports
Researchers identified Klebsiella phage 32 RNA polymerase as an alternative to T7 for RNA production. The enzyme tolerated a broad range of temperatures, pH values, and salt conditions. For an 11,551-nucleotide self-amplifying RNA retaining its native AU initiation, production was 9.3-fold higher than the T7 comparison. These laboratory results do not establish GMP-scale performance.
Why this paper is included
Highlights transcription options for long saRNA constructs; scale-up requires evaluating impurity profiles, capping compatibility, process robustness, and batch consistency.
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