Biotech Assistant
Share useful research with your peers
in · Share on LinkedIn ↗
RESEARCH / LITERATURE

Research Literature

Find research, methods, and reviews across our scientific fields.

One collection · Original publication dates · Authors, journals & DOIs

mRNA / LNP 70

Duplicate DOIs are merged. This is a selected collection, not an exhaustive bibliography.

Clear filtersExport this page · RIS ↓
Original research
Nature Materials

In situ CAR engineering and nanoparticle-mediated TREM2 degradation for macrophage reprogramming in malignant ascites

Yijia Li; Ting Yang; Xiyue Zhang; Rui Peng; Jifang Gong; Zihan Zhang; Bin Ma; Yu Hou; Yunxuan Liu; Kemeng Chen; Qidong Bai; Yian Fang; Changdi Cao; Jia Hao; Zhiwei Sun; Ziqiong Jiang; Mengying Dong; Chenlong Wei; Zhongtang Li; Qiang Zhang; Lin Shen; Xueqing Wang; Mi Deng; Changsong Qi; Lei Miao

A Nature Materials study describes mRNA-loaded LNPs that reprogram tumor-associated macrophages into Claudin18.2-targeting CAR macrophages. The lipid formulation also promotes TREM2 internalization and degradation, reshaping immune responses. The original abstract reports tumor suppression and longer survival with PD-1 blockade in mice, plus ex vivo patient-sample findings. These findings do not demonstrate clinical efficacy in treated patients.

mRNA / LNPIn Vivo Cell Engineering & Gene EditingProtein degradation / PROTAC
Read guide →Original paper ↗

DOI: 10.1038/s41563-026-02769-2

Original research
Nature Nanotechnology

Rational design of lipid nanoparticles for brain delivery of RNA therapeutics

Meng Tian; Leiming Wang; Zhengwei Liu; Dinglingge Cao; Changyue Yu; Jeffrey L. Bennett; Eliza Bliss-Moreau; Chang Wang; Diana D. Kang; Yonger Xue; Yichen Zhong; Siyu Wang; Haoyuan Li; Xucheng Hou; Binbin Deng; Yizhou Dong

Researchers used the properties of more than 100 central nervous system drugs to design brain-directed ionizable lipids. HX1 nanoparticles delivered RNA and enabled gene editing in mouse brains, delivered reporter mRNA in a nonhuman primate, and reduced radiation-related brain injury in mice carrying Il10 mRNA. These are preclinical findings.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41565-026-02326-7

Original research
Nature Communications

AI-driven discovery and engineering of human-derived protein scaffolds for mRNA delivery

Dongmin Xun; Lanqing Li; Jie Zou; Yi Liu; Ziyue Yang; Qianyi Liang; Ke Li; Zhu Zhu; Jicong Zhang; Lijun Lang; Kun Liu; Xinyu Wang; Meilin Tian; Guangyong Chen; Jun Yang; Bolin An; Pheng Ann Heng; Chao Zhong

The DeepDelivery platform screened the human proteome for proteins that could serve as mRNA delivery scaffolds. Twelve of 15 experimentally tested candidates supported particle formation and delivery within a modular system. Engineering TRIM25 domains improved performance. The work establishes a discovery framework in cultured cells, rather than clinical evidence of reduced immunogenicity.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41467-026-79039-x

Methods
International Journal of Pharmaceutics

Simple heat-assisted method for mRNA loading into empty lipid nanoparticles enables robust in vitro and in vivo delivery

Takayuki Miyazaki; Kenji Kubara; Keita Kondo; Hiroshi Kamiyama; Shoki Hara; Jun Moriya; Hiroshi Ishihara; Yuta Suzuki

A post-loading method combined mRNA with preformed empty lipid nanoparticles using heat and acidic conditions, achieving roughly 90% encapsulation. The particles produced protein expression in mouse liver and spleen, although activity was slightly below conventional microfluidic formulations. Greater payload heterogeneity emerged as a potential limitation; the method supports 96-well preparation.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.ijpharm.2026.127599

Original research
Journal of controlled release : official journal of the Controlled Release Society

Overcoming technological and biological barriers to pulmonary mRNA delivery with lipid-polymer hybrid nanoparticles

Biesel A; Shandheep A; Pavlou A; Busker LM; Koch M; Kopliku V; Maisel K; Boese A; Skerra J; Schröder LJ; Neubert L; Schneider-Daum N; Loretz B; Kalinke U; Lehr CM

Lipid-polymer hybrid particles combined polymer stability with lipid-mediated transfection. Hyaluronic acid and polyvinyl alcohol coatings helped preserve particle properties after nebulization, while mucus-covered airway cultures retained measurable transfection. Primary human lung fibroblasts and intratracheally dosed mice provided additional delivery evidence. These results support aerosol formulation development, although intratracheal dosing does not establish the performance of inhaled aerosols in patients.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.jconrel.2026.115509

Original research
Nature Biotechnology

An mRNA–lipid nanoparticle vaccine against H5N1 influenza virus protects lactating dairy cows from infection

Jefferson J. S. Santos; Carine K. Souza; Giovana C. Zanella; Débora B. Goulart; Bailey Arruda; Paola Boggiatto; Mitchell V. Palmer; Celeste A. Snyder; Michaela A. Kristula; Charlene Dickens; Terry L. Webb; Reilly K. Atkinson; Bernadeta Dadonaite; Garima Dwivedi; Mohamad-Gabriel Alameh; Christopher A. Hunter; Lisa A. Murphy; Jesse D. Bloom; Drew Weissman; Gary C. Althouse; Amy L. Baker; Scott E. Hensley

An experimental H5N1 mRNA-LNP vaccine was tested in eight lactating dairy cows split between vaccinated and control groups. After two doses and mammary challenge, vaccination reduced peak viral RNA in milk by about 1,000-fold and lessened mastitis and fibrosis. This small animal study does not establish field effectiveness or human protection.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41587-026-03343-6

Original research
Journal of controlled release : official journal of the Controlled Release Society

Uncovering the lymphatic uptake, pharmacokinetics, and biodistribution of mRNA-lipid nanoparticles after intravenous and subcutaneous injection

Peng Li; Mohammad Abdallah; Yuxin Yao; Rekha Shandre Mugan; Thomas J. Payne; Stewart A. Fabb; Colin W. Pouton; Natalie L. Trevaskis

Using three established ionizable lipids, researchers measured RNA, lipid components, lymphatic transport, plasma exposure, and tissue reporter expression in rats. After subcutaneous injection, lymph concentrations exceeded plasma concentrations, but lipid and RNA trafficking did not fully match. The study demonstrates why tracking a lipid label alone may not accurately represent intact RNA delivery or productive expression in tissues.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.jconrel.2026.115491

Reviews & perspectives
Nature Reviews Materials

Programmable mRNA vaccine materials for temporal control of antitumour immunity

Seong Dong Jeong; Chaoyang Tang; Xiaotian Wang; Antoni J. Szeglowski; Yujin Kim; B. J. Kim; Padmanee Sharma; James J. Moon; Wen Jiang; Betty Y. S. Kim

A Perspective proposes designing mRNA vaccine materials around changing immune conditions before and after cancer surgery. The framework considers a broadly applicable preoperative prime followed by a personalized postoperative boost, with carriers controlling expression and immune activation. It is a conceptual design roadmap, not evidence from a new clinical trial.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s41578-026-00974-8

Original research
Molecular therapy : the journal of the American Society of Gene Therapy

A Scalable, Antibody-Free Lipid Nanoparticle Achieves Preferential mRNA Delivery to Human HSPCs In Vivo Without Conditioning

Kim E; Zenhausern R; Kapadia S; Johnson S; Cho S; Choi J; Park K; Diaz E; Podilapu AR; Kuppan R; Dahlman JE; Kim H; Woo J

A ligand-free nanoparticle formulation preferentially delivered mRNA to human hematopoietic stem and progenitor cells in humanized mice without conditioning. It reduced hepatic delivery relative to an SM-102 comparator and enabled functional Cas9-mediated editing of human CD34-positive cells. Single-cell analyses supported tolerability under the conditions examined. Durable stem-cell function and clinical delivery still require further investigation.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.ymthe.2026.10.024

Original research
Molecular therapy : the journal of the American Society of Gene Therapy

A Combinatorial mRNA Therapy for Treating Rheumatoid Arthritis and Osteoarthritis by Inhibiting Inflammation and Preventing Cartilage Degeneration

Wang G; Guo Y; Guo K; Zhang C; Zhou Y; Xia J; Jiang H; Liu J; Ren J; Mohamed AO; Tang K; Chen X; Sun S; Xing Y; Yang Y; Shen M; Wang R; Huang Y; Li X; Lin A

An engineered nanoparticle delivered mRNAs encoding IL-1 receptor antagonist and an angiopoietin-like 3 derivative through intra-articular injection. The combination addressed inflammation and cartilage degeneration in several mouse arthritis models, with transcriptomic analyses supporting complementary biological effects. These findings broaden local mRNA therapy beyond vaccination, but durable structural benefit, repeated-dose safety, and effectiveness in human joints remain unproven.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.ymthe.2026.10.021

Reviews & perspectives
Journal of Pharmaceutical Sciences

Particle occupancy in mRNA lipid nanoparticles: Measurement, manufacturing determinants, and relevance to product quality

Carlos Victor Montefusco-Pereira

A minireview distinguishes bulk encapsulation efficiency from particle occupancy and RNA copy-number distributions. High encapsulation efficiency can coexist with RNA-negative particles, and process changes may alter loading without changing average size. The author cautions that eliminating empty particles does not necessarily improve activity; product-specific evidence must establish whether occupancy is a critical quality attribute.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1016/j.xphs.2026.104556

Original research
EMBO Molecular Medicine

Intramuscular self-amplifying RNA and adenoviral RSV vaccines program distinct systemic and respiratory mucosal immune architectures

Yao Zhang; Yu Qin; Zhongbing Qi; Qingzhe Yang; Xian Zhang; Shichuan Hu; Yuejun Li; Boduan Xiao; Jianchuan Hu; Ping Cheng

A mouse study compared LNP-delivered self-amplifying RNA and an Ad5 vaccine encoding the same RSV prefusion F antigen. Intramuscular dosing produced distinct immune patterns: saRNA favored pulmonary mucosal and tissue-resident memory responses, while Ad5 generated stronger systemic responses. The comparison concerns immune architecture, not demonstrated superiority in human protection.

mRNA / LNP
Read guide →Original paper ↗

DOI: 10.1038/s44321-026-00537-y

AI assistant ↗